Fundamental to Primity's assay platforms is the concept of "single cell analysis", whereby each cell in a sample is measured and analyzed. This reveals the true distribution of cellular phenotypes, and is not susceptible to the outliers or heterogeneous populations that plague typical "total well" assay formats.
Measure 10+ readouts/markers per cell. Analyze 5,000 cells per second. Obtain sensitivity down to ~50 molecules per cell. Not many platforms can compete with flow cytometry when it comes to single cell analysis. Traditionally used by immunologists, we are pioneers in using cytometry for drug discovery and multiplexed analysis of compound activity.
A core competency at Primity is analysis of primary blood samples, whether they are isolated PBMCs, or whole blood. We have extensive experience in processing blood samples optimally for various surface and intracellular targets, from activation markers to phospho-proteins and cytokines. We understand what it takes to make an assay robust enough for clinical application, and can perform stability and precision validation for your exploratory clinical trial endpoints.
One of the challenges with flow cytometry is that protocols often require washing cells multiple times and adding several different buffers and reagents. At Primity, we have built a state-of-the-art automation system capable of performing even the most complex cell protocols, including phospho-specific flow cytometry. This lets us run miniaturized 384 well plate assays that leads to lower reagent usage, more robust data, and a unique ability to run ~2,000 compound wells per day to generate over 100,000 data points using our CellCode multiplexing technology.
Acquiring 10,000 data points is nice, but if you don't know what to do with it, it can be challenging. At Primity, we employ advanced visualizations to interpret data, harnessing the combined power of Cytobank flow cytometry software and Spotfire analytics software. Using these tools, we can rapidly move from millions of cellular events to actionable visualizations of compound activity across 100's of readouts.